16S rRNA sequencing of samples from universal stool bank donors
نویسندگان
چکیده
منابع مشابه
Genetic Characterization of Argas persicus From Iran by Sequencing of Mitochondrial Cytochrome Oxidase I (COX1) and 16s rRNA Genes
Background: Argas persicus has a great importance for health and veterinary, it can transmit many infectious agents such as Borrelia anserina (avian spirochetosis) and Aegyptianella pullorum. Distinguishing Argasidae due to close morphological relationship is difficult. OBJECTIVES: In the present study, we performed molecular analyses based on PCR and sequencing of Amplicon derived from 16S rRN...
متن کاملPCR Amplification, Sequencing of 16S rRNA Genes with Universal Primers and Phylogenetic Analysis of Pseudomonas aeruginosa
Pseudomonas aeruginosa is a gram negative bacterium isolated from soil. The isolate was cultured in a selective medium (Pseudomonas Agar base) at 37oC for 24 hours. For species identification, Pseudomonas like organisms creates lot of problems when identified with the help of morphological and biochemical characters. However, sequencing of 16S rRNA region is a suitable technique for species ide...
متن کاملRapid 16S rRNA Next-Generation Sequencing of Polymicrobial Clinical Samples for Diagnosis of Complex Bacterial Infections
Classifying individual bacterial species comprising complex, polymicrobial patient specimens remains a challenge for culture-based and molecular microbiology techniques in common clinical use. We therefore adapted practices from metagenomics research to rapidly catalog the bacterial composition of clinical specimens directly from patients, without need for prior culture. We have combined a semi...
متن کاملModelling Computational Resources for Next Generation Sequencing Bioinformatics Analysis of 16S rRNA Samples
In the rapidly evolving domain of next generation sequencing and bioinformatics analysis, data generation is one aspect that is increasing at a concomitant rate. The burden associated with processing large amounts of sequencing data has emphasised the need to allocate sufficient computing resources to complete analyses in the shortest possible time with manageable and predictable costs. A novel...
متن کاملResearch benefits of storing genitourinary samples: 16S rRNA sequencing to evaluate vaginal bacterial communities
Research benefits of storing genitourinary samples: 16S rRNA sequencing to evaluate vaginal bacterial communities Dear Editor, Using well-characterised, but old and carefully frozen genital tract research samples may be a cost-effective way of performing metagenomic studies, but risks loss of low abundance (but relevant) bacterial species DNA. Moi et al. 1 used 16S rRNA and UreDNA sequencing to...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: BMC Research Notes
سال: 2021
ISSN: 1756-0500
DOI: 10.1186/s13104-021-05520-z